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Knockdown of CNGB3 suppressed the intracellular Ca 2+ concentration and upregulated the expression of MHC-I in GC cells. (A,B) Intracellular Ca 2+ levels following CNGB3 gene knockdown. Confocal illustration of intracellular Ca 2+ imaging (A) and quantification (B). Scale bar: 100 μm (staining <t>method:</t> <t>Fluo-4</t> AM staining; magnification: 100×). (C-E) Expression level of MHC-I molecules in cells after CNGB3 gene knockdown. **, P<0.01; ***, P<0.001. GC, gastric cancer; MHC-I, major histocompatibility complex class I.
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Knockdown of CNGB3 suppressed the intracellular Ca 2+ concentration and upregulated the expression of MHC-I in GC cells. (A,B) Intracellular Ca 2+ levels following CNGB3 gene knockdown. Confocal illustration of intracellular Ca 2+ imaging (A) and quantification (B). Scale bar: 100 μm (staining <t>method:</t> <t>Fluo-4</t> AM staining; magnification: 100×). (C-E) Expression level of MHC-I molecules in cells after CNGB3 gene knockdown. **, P<0.01; ***, P<0.001. GC, gastric cancer; MHC-I, major histocompatibility complex class I.
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Fstl1 enhances the osteogenic differentiation potential of MC3T3-E1 cells. (A) Representative alkaline-phosphatase (ALP) staining at 7 d. (B) Quantitative ALP activity. (C) Representative Alizarin Red S (ARS) staining at 14 d. (D) Quantification of ARS absorbance (OD 562 ). (E-H) Relative mRNA expression of Runx2, Sp7, Ocn, and Opn at 14 d determined by RT-qPCR. (I) Western-blot bands for RUNX2 and SP7. (J and K) Densitometric ratios of RUNX2/GAPDH and SP7/GAPDH. Sample sizes: con ( n = 3), OE-Fstl1 ( n = 3), and sh-Fstl1 ( n = 3). Data are presented as mean ± SD; inter-group comparisons were performed using 1-way ANOVA. * p < .05, ** p < .01, *** p < .001, **** p < .0001; ns, not significant.

Journal: JBMR Plus

Article Title: Follistatin-like protein 1 (FSTL1) modulates bone remodeling and attenuates bone loss in a mouse model of postmenopausal osteoporosis

doi: 10.1093/jbmrpl/ziag037

Figure Lengend Snippet: Fstl1 enhances the osteogenic differentiation potential of MC3T3-E1 cells. (A) Representative alkaline-phosphatase (ALP) staining at 7 d. (B) Quantitative ALP activity. (C) Representative Alizarin Red S (ARS) staining at 14 d. (D) Quantification of ARS absorbance (OD 562 ). (E-H) Relative mRNA expression of Runx2, Sp7, Ocn, and Opn at 14 d determined by RT-qPCR. (I) Western-blot bands for RUNX2 and SP7. (J and K) Densitometric ratios of RUNX2/GAPDH and SP7/GAPDH. Sample sizes: con ( n = 3), OE-Fstl1 ( n = 3), and sh-Fstl1 ( n = 3). Data are presented as mean ± SD; inter-group comparisons were performed using 1-way ANOVA. * p < .05, ** p < .01, *** p < .001, **** p < .0001; ns, not significant.

Article Snippet: On day 21, ARS staining was carried out with an Alizarin Red S calcium-staining kit (Beyotime Biotechnology): after fixation, working solution was applied, and the cells were incubated for 30 min at room temperature in the dark, and the reaction was terminated once red mineralized nodules became visible.

Techniques: Staining, Activity Assay, Expressing, Quantitative RT-PCR, Western Blot

Knockdown of CNGB3 suppressed the intracellular Ca 2+ concentration and upregulated the expression of MHC-I in GC cells. (A,B) Intracellular Ca 2+ levels following CNGB3 gene knockdown. Confocal illustration of intracellular Ca 2+ imaging (A) and quantification (B). Scale bar: 100 μm (staining method: Fluo-4 AM staining; magnification: 100×). (C-E) Expression level of MHC-I molecules in cells after CNGB3 gene knockdown. **, P<0.01; ***, P<0.001. GC, gastric cancer; MHC-I, major histocompatibility complex class I.

Journal: Translational Cancer Research

Article Title: Identification of a novel ion channel-related gene signature to predict prognosis and immune response of gastric cancer

doi: 10.21037/tcr-2025-1-2874

Figure Lengend Snippet: Knockdown of CNGB3 suppressed the intracellular Ca 2+ concentration and upregulated the expression of MHC-I in GC cells. (A,B) Intracellular Ca 2+ levels following CNGB3 gene knockdown. Confocal illustration of intracellular Ca 2+ imaging (A) and quantification (B). Scale bar: 100 μm (staining method: Fluo-4 AM staining; magnification: 100×). (C-E) Expression level of MHC-I molecules in cells after CNGB3 gene knockdown. **, P<0.01; ***, P<0.001. GC, gastric cancer; MHC-I, major histocompatibility complex class I.

Article Snippet: Cellular Ca 2+ levels were measured using a Fluo-4 AM Fluorescence Calcium Ion Detection Kit (Servicebio, Wuhan, China).

Techniques: Knockdown, Concentration Assay, Expressing, Imaging, Staining, Immunopeptidomics